Journal: Kidney Diseases
Article Title: The Effect of Low-Density Lipoprotein Receptor-Related Protein-1 on Acute Kidney Injury and Renal Tubular Epithelial Triglyceride Accumulation
doi: 10.1159/000545851
Figure Lengend Snippet: RAP improved IRI induced AKI and reduced renal triglyceride accumulation. a PAS staining showed RAP improved renal tubular epithelial cell injury (200×). b Quantification of tubular injury in each group. Serum creatinine ( c ) and BUN ( d ) levels were measured in each group. e , f Oil Red O staining showed RAP reduce neutral lipid accumulation in renal tubules (400×). g Serum triglyceride was measured in each group. Measurement of renal cortex triglycerides ( h ), cholesterol ester ( i ), and renal cortex FFA ( j ) in each group. k immunostaining for LRP1(red), LTL(green), and DAPI (blue) was used in frozen renal sections in each group (200×). l , m Western blot was conducted to assess the protein levels of LRP1. n qPCR was used to detect the mRNA level of LRP1. * p < 0.05. White arrow indicates LRP1 expression at the brush border of proximal tubules; white triangle indicates LRP1 expression at the basolateral side of proximal renal tubular epithelial cells. BUN, blood urea nitrogen; LRP1, low-density lipoprotein receptor-related protein-1; FFA, free fatty acid; IRI, ischemia-reperfusion injury; LTL, lotus tetragonolobus lectin; RAP, receptor-associated protein.
Article Snippet: A specific quantity of tissues was weighed to prepare the homogenate, and then TG Content Assay Kit (Cat. No. BC0625, Solarbio, Beijing, China), Amplex Red Cholesterol and Cholesteryl Ester Assay Kit (Cat. No. S0211M, Beyotime, Shanghai, China), and Free Fatty Acids (FFAs) Content Assay Kit (Cat. No. BC0595, Solarbio, Beijing, China) were used to assess the content of lipids in tissues according to the instructions provided.
Techniques: Staining, Immunostaining, Western Blot, Expressing